with 9 state-of-the-art technological platforms
Seminar Hervé Rigneault (Inst. Fresnel), 26 June 2025 11am on "Multiphoton and coherent Raman imaging from microscopy to endoscopy"
Seminar 26 June 2025 at 11.00am in the ICM Auditorium.
Multiphoton and coherent Raman imaging from microscopy to endoscopy
Hervé Rigneault
Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France
Lightcore technologies, Marseille, France
I will present advances in the field of nonlinear microscopy and endoscopy to perform multiphoton and label free images using nonlinear contrast mechanisms.
From the microscopy side I will present coherent Raman imaging, a microscopy technique that enables label free imaging. Coherent Raman scattering encompasses coherent anti-Stokes Raman scattering (CARS), coherent Stokes Raman scattering (CSRS) and stimulated Raman scattering (SRS) [1]. In this talk I will review how coherent Raman processes generate virtual histology images in real time without any sample preparation of brain samples [2] and live organoid [3] and cells.
From the endoscopy side, I will present our effort to perform multiphoton imaging (including coherent Raman) in endoscopy using innovative hollow core optical fibers and miniature piezo scanners [4]. I will present the first results using the endoscope to perform brain imaging in awaken freely moving mice [5].
These academic developments have led to the creation of a company, Lightcore Technologies (https://lightcore-technologies.com/) that commercializes both the multiphoton and coherent Raman microscope and the multiphoton endoscope.
References:
[1] H. Rigneault, and P. Berto, “Tutorial: Coherent Raman light matter interaction processes,” APL Photonics 3, 091101 (2018).
https://doi.org/10.1063/1.5030335
[2] R. Appay, B. Sarri, S. Heuke, S. Boissonneau, C. Liu, E. Dougy, L. Daniel, D. Scavarda, H. Dufour, D. Figarella-Branger, and H. Rigneault, “Live Stimulated Raman Histology for the Near-Instant Assessment of Central Nervous System Samples,” The Journal of Physical Chemistry B 127, 3624-3631 (2023).
https://pubs.acs.org/doi/10.1021/acs.jpcb.3c01156
[3] B. Sarri, V. Chevrier, F. Poizat, S. Heuke, F. Franchi, L. De Franqueville, E. Traversari, J.-P. Ratone, F. Caillol, Y. Dahel, S. Hoibian, M. Giovannini, C. de Chaisemartin, R. Appay, G. Guasch, and H. Rigneault, “In vivo organoid growth monitoring by stimulated Raman histology,” npj Imaging 2, 18 (2024).
[4] A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Science & Applications 7, 10 (2018).